This suggests that coating the surface of EC was successful in protecting bacteria from the environmental stressors. Although coating has a negative effect on the preservation of bacterial density under normal conditions, the bacterial density was higher for coated EC than for uncoated EC ( 2.4 × 10 3 CFU / g of EC) when exposed to a harsh environment (60☌ and pH 12) for 48 h. First, the presence of bacteria ( Lysinibacillus boronitolerans YS11) within EC was verified using electron microscopy after the bacteria were immobilized. In this study, self-healing performance by EC was examined using styrene-acrylic emulsion coatings.
Using expanded clay (EC) as a carrier is attractive because it provides adequate bond strength to the cement composites as well as protection to the bacteria from harsh environments.
To overcome the harsh environments of concrete, various carriers have been tested for their ability to protect the healing bacteria. Concrete crack healing by microbial calcium carbonate producers has been intensively studied for the last 10 years.
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